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ScienCell pericyte growth medium (pgm
a, Schematic of microwell plates in 96-well plate format and representative phase contrast image of a GRi3D organoid array within a single well. Scale bar, 1 mm. b, Quantification of blood-brain barrier organoid diameter in GRi3D microwell arrays within 24- or 96-well plate compared to organoids grown on agarose substrate. Each dot represents a single organoid, while different colors represent independent experiments. c, Representative confocal images of a blood-brain barrier organoid labelled with cell type-specific markers showing specific layering and organization of brain endothelial cells (P-gp, magenta), <t>pericytes</t> (NG2, green) and astrocytes (GFAP, cyan) when grown in GRi3D arrays. Nuclei are labelled with DAPI (grey). Scale bars, 100 μm. d, Representative fluorescent image of a live/dead staining showing the viability of blood-brain barrier organoids grown on GRi3D arrays. Calcein AM in green labels live cells whereas ethidium homodimer-1 in magenta labels dead cells. Scale bar, 1 mm. Images on the right show a higher magnification of the boxed area. Scale bar 500 μm.
Pericyte Growth Medium (Pgm, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pericyte+growth+medium+(pgm/pericyte+growth+medium/bio_rxiv__2021__02__09__430382-35-17-20
Average 90 stars, based on 1 article reviews
pericyte growth medium (pgm - by Bioz Stars, 2026-09
90/100 stars

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1) Product Images from "Investigating receptor-mediated antibody transcytosis using Blood-Brain Barrier organoid arrays"

Article Title: Investigating receptor-mediated antibody transcytosis using Blood-Brain Barrier organoid arrays

Journal: bioRxiv

doi: 10.1101/2021.02.09.430382

a, Schematic of microwell plates in 96-well plate format and representative phase contrast image of a GRi3D organoid array within a single well. Scale bar, 1 mm. b, Quantification of blood-brain barrier organoid diameter in GRi3D microwell arrays within 24- or 96-well plate compared to organoids grown on agarose substrate. Each dot represents a single organoid, while different colors represent independent experiments. c, Representative confocal images of a blood-brain barrier organoid labelled with cell type-specific markers showing specific layering and organization of brain endothelial cells (P-gp, magenta), pericytes (NG2, green) and astrocytes (GFAP, cyan) when grown in GRi3D arrays. Nuclei are labelled with DAPI (grey). Scale bars, 100 μm. d, Representative fluorescent image of a live/dead staining showing the viability of blood-brain barrier organoids grown on GRi3D arrays. Calcein AM in green labels live cells whereas ethidium homodimer-1 in magenta labels dead cells. Scale bar, 1 mm. Images on the right show a higher magnification of the boxed area. Scale bar 500 μm.
Figure Legend Snippet: a, Schematic of microwell plates in 96-well plate format and representative phase contrast image of a GRi3D organoid array within a single well. Scale bar, 1 mm. b, Quantification of blood-brain barrier organoid diameter in GRi3D microwell arrays within 24- or 96-well plate compared to organoids grown on agarose substrate. Each dot represents a single organoid, while different colors represent independent experiments. c, Representative confocal images of a blood-brain barrier organoid labelled with cell type-specific markers showing specific layering and organization of brain endothelial cells (P-gp, magenta), pericytes (NG2, green) and astrocytes (GFAP, cyan) when grown in GRi3D arrays. Nuclei are labelled with DAPI (grey). Scale bars, 100 μm. d, Representative fluorescent image of a live/dead staining showing the viability of blood-brain barrier organoids grown on GRi3D arrays. Calcein AM in green labels live cells whereas ethidium homodimer-1 in magenta labels dead cells. Scale bar, 1 mm. Images on the right show a higher magnification of the boxed area. Scale bar 500 μm.

Techniques Used: Staining



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ScienCell pericyte growth medium (pgm
a, Schematic of microwell plates in 96-well plate format and representative phase contrast image of a GRi3D organoid array within a single well. Scale bar, 1 mm. b, Quantification of blood-brain barrier organoid diameter in GRi3D microwell arrays within 24- or 96-well plate compared to organoids grown on agarose substrate. Each dot represents a single organoid, while different colors represent independent experiments. c, Representative confocal images of a blood-brain barrier organoid labelled with cell type-specific markers showing specific layering and organization of brain endothelial cells (P-gp, magenta), <t>pericytes</t> (NG2, green) and astrocytes (GFAP, cyan) when grown in GRi3D arrays. Nuclei are labelled with DAPI (grey). Scale bars, 100 μm. d, Representative fluorescent image of a live/dead staining showing the viability of blood-brain barrier organoids grown on GRi3D arrays. Calcein AM in green labels live cells whereas ethidium homodimer-1 in magenta labels dead cells. Scale bar, 1 mm. Images on the right show a higher magnification of the boxed area. Scale bar 500 μm.
Pericyte Growth Medium (Pgm, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pericyte+growth+medium+(pgm/pericyte+growth+medium/bio_rxiv__2021__02__09__430382-35-17-20
Average 90 stars, based on 1 article reviews
pericyte growth medium (pgm - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


a, Schematic of microwell plates in 96-well plate format and representative phase contrast image of a GRi3D organoid array within a single well. Scale bar, 1 mm. b, Quantification of blood-brain barrier organoid diameter in GRi3D microwell arrays within 24- or 96-well plate compared to organoids grown on agarose substrate. Each dot represents a single organoid, while different colors represent independent experiments. c, Representative confocal images of a blood-brain barrier organoid labelled with cell type-specific markers showing specific layering and organization of brain endothelial cells (P-gp, magenta), pericytes (NG2, green) and astrocytes (GFAP, cyan) when grown in GRi3D arrays. Nuclei are labelled with DAPI (grey). Scale bars, 100 μm. d, Representative fluorescent image of a live/dead staining showing the viability of blood-brain barrier organoids grown on GRi3D arrays. Calcein AM in green labels live cells whereas ethidium homodimer-1 in magenta labels dead cells. Scale bar, 1 mm. Images on the right show a higher magnification of the boxed area. Scale bar 500 μm.

Journal: bioRxiv

Article Title: Investigating receptor-mediated antibody transcytosis using Blood-Brain Barrier organoid arrays

doi: 10.1101/2021.02.09.430382

Figure Lengend Snippet: a, Schematic of microwell plates in 96-well plate format and representative phase contrast image of a GRi3D organoid array within a single well. Scale bar, 1 mm. b, Quantification of blood-brain barrier organoid diameter in GRi3D microwell arrays within 24- or 96-well plate compared to organoids grown on agarose substrate. Each dot represents a single organoid, while different colors represent independent experiments. c, Representative confocal images of a blood-brain barrier organoid labelled with cell type-specific markers showing specific layering and organization of brain endothelial cells (P-gp, magenta), pericytes (NG2, green) and astrocytes (GFAP, cyan) when grown in GRi3D arrays. Nuclei are labelled with DAPI (grey). Scale bars, 100 μm. d, Representative fluorescent image of a live/dead staining showing the viability of blood-brain barrier organoids grown on GRi3D arrays. Calcein AM in green labels live cells whereas ethidium homodimer-1 in magenta labels dead cells. Scale bar, 1 mm. Images on the right show a higher magnification of the boxed area. Scale bar 500 μm.

Article Snippet: Human brain microvascular pericytes (HBVP, ScienCell Research Laboratories) were cultured in Pericyte Growth Medium (PGM) composed of Pericytes Medium (PM, ScienCell Research Laboratories) supplemented with 2% FBS, 1% Pericytes Growth Supplement (PGS, ScienCell Research Laboratories) and 1% penicillin/streptomycin.

Techniques: Staining